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Thermodynamic and kinetic characterizations of the RMR1-mediated diazaborine formation. (a) A dilution experiment demonstrating the reversibility of RMR1-lysine conjugation. (b) UV-vis absorption changes of RMR1 upon lysine conjugation. (c) A titration experiment revealing the Kd value of RMR1-lysine conjugation. (d) Dissociation kinetics of the diazaborine conjugate of RMR1 and lysine. (e) Tabulated thermodynamic and kinetic parameters of RMR1 conjugation with small molecule amines.
