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. 2022 Mar 17;18:102. doi: 10.1186/s12917-022-03163-1

Table 1.

Effects of weaning age and parity on ammonia, VFA, and pH in rumen fluid evaluated by two-way ANOVA on experimental d 140. Values are presented as Means with SD

Early-weaning Late-weaning p-values
Parameters MC PC MC PC Weaning age W Parity P W*P
 Ammonia-N (mg/100 g)

2.33

 ± 1.25

1.91

 ± 1.71

2.93

 ± 2.07

2.52

 ± 1.18

0.144 0.289 1

 Acetate

 (mmol/L)

35.89

 ± 6.71

37.99

 ± 8.23

36.15

 ± 6.78

38.06

 ± 5.88

0.971 0.278 0.917
 Propionate (mmol/L)

9.44

 ± 1.91

10.42

 ± 2.18

9.91

 ± 2.38

10.65

 ± 2.89

0.605 0.170 0.723

 Butyrate

 (mmol/L)

5.13

 ± 1.67

5.76

 ± 1.67

5.11

 ± 1.50

5.52

 ± 2.00

0.741 0.234 0.876
 Iso-Butyrate (mmol/L) 0.66 ± 0.15 0.74 ± 0.16 0.67 ± 0.15 0.74 ± 0.20 0.948 0.099 0.974

 Valerate

 (mmol/L)

0.70

 ± 0.19

0.82

 ± 0.21

0.74

 ± 0.24

0.72

 ± 0.22

0.630 0.337 0.238
 Iso-Valerate (mmol/L) 1.10 ± 0.52 1.36 ± 0.55 0.99 ± 0.22 1.15 ± 0.46 0.172 0.078 0.665
 Total VFA (mmol/L)

52.92

 ± 10.25

57.10

 ± 12.12

53.57

 ± 10.23

56.84

 ± 10.22

0.990 0.189 0.856
 Ratio acetate:propionate

3.84

 ± 0.47

3.67

 ± 0.44

3.74

 ± 0.60

3.69

 ± 0.56

0.781 0.435 0.643
 pH

7.34

 ± 0.21

7.32

 ± 0.27

7.35

 ± 0.21

7.32

 ± 0.17

0.857 0.684 0.960