Fig. 4.

Characterization of the novel GH10 xylanase Pm25 and its mutants. Composites were incubated in 0.03 mg/ mL wild-type Pm25, 0.03 mg/mL variant M5 (Pm25 Y213A + Y378A), 0.017 mg/ mL variant M6 (Pm25∆CBMs), 0.03 mg/ mL variant M1 (Pm25 E546A), or buffer only, at pH 5.0, 40 °C and 300 rpm for 3–20 h. A Representative cellulose/azo-xylan composites dried after treatment. B Average intensity values of dried cellulose/azo-xylan composites per segment. C Absorbance of the reaction solution at 590 nm recovered after composite incubation; statistically significant differences compared to the wild-type Pm25 are indicated by an asterisk (two-tailed t-test; p ≤ 0.05). For all experiments, n ≥ 4; error bars correspond to standard deviation of mean