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. 2022 Mar 16;15:30. doi: 10.1186/s13068-022-02128-8

Table 1.

Properties of enzymes used in this study

Enzyme Organism CAZY number Molecular mass (kDa) CBM UNIPROT Accession number Specific activity (U/mg)*
CmXyn10B Cellvibrio mixtus GH10 41.7 O68541 7.32
NpXyn11A Neocallimastix patriciarum GH11 25.8 P29127 14.2
TfXyn11A Thermobifida fusca GH11 31.9 CBM2b Q47QL8 8.06
Pm25 (WT) Unclassified; from termite gut metagenome GH10 83.9 CBM4 S0DFK9 0.29
Pm25 Y213A Y378A (M5) 83.8 CBM4 0.29
Pm25∆CBMs (M6) 48.8 0.49
Pm25 E546A (M1) 83.9 CBM4 0.01

*Determined using azo-xylan at pH 5.0 and 40 °C