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The immunocytochemical evaluation of HSP27 expression in: (A) H9C2 cells, (B) HCM cells
| Incubation time [h] | Custodiol HTK | Plegisol | Control cells |
|---|---|---|---|
| (A) | |||
| 0.5 |
< 5%* −/+ |
< 5%* + |
45% ++ |
| 1 |
18%* + |
20%* + |
|
| 2 |
20–12% + |
40% + |
|
| 4 |
25%* + |
70%* +/++ |
|
| (B) | |||
| 0.5 | No reaction |
<5% + |
45% ++ |
| 1 | No reaction |
30% +/++ |
|
| 2 | No reaction |
50%* +/++ |
|
| 4 |
< 5%* +/++ |
no reaction | |
*For p ≤ 0.05
