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. Author manuscript; available in PMC: 2022 Mar 17.
Published in final edited form as: Cell. 2022 Jan 3;185(2):361–378.e25. doi: 10.1016/j.cell.2021.12.015

Figure 4. A double outer ring in a subset of yeast NPCs.

Figure 4.

(A) The isolated yeast NPC with a double outer ring segmented into proximal (gold) and distal (tan) rings. Density for Nup188/192 (red) and two orphans (silver) are shown. A double ring protomer is outlined (dashed box).

(B) Model of Y-complexes in the double outer ring with the overlaid density map.

(C) Top: three pairs of Y-complexes from the 3D density map. A gap is visible between neighboring Y-complexes in the distal ring (tan). Bottom: molecular models are shown in the map with MBMs indicated (*).

(D) Y-complexes from the distal (left) and proximal (center) outer rings are shown, along with a staggered pair (right). A torus-like bulge and Nup133 spur are present in the tails of distal and proximal Y-complexes, respectively.

(E) The pair of Y-complexes outlined in Figure 4A is shown with density for a Nup188/192 molecule (red) and three orphans (numbered 1–3). Orphans, MBMs(*), and a tripod of β-propellers are labeled. Scale bars are indicated on the panels.