Table 1.
Primers and siRNA sequences.
| Name | Species | Forward (5′ to 3′) | Reverse (5′ to 3′) |
|---|---|---|---|
| SHH | Human | GGACAGGCTGATGACTCAGA | GCCCTCGTAGTGCAGAGACT |
| Ptch1 | Mouse | CTCTGGAGCAGATTTCCAAGG | TGCCGCAGTTCTTTTGAATG |
| SMO | Mouse | CCTGACTTTCTGCGTTGC | GGTTCTGACACTGAATCCC |
| SUFU | Mouse | GCTTTGAGTTGACGTTTCGT | CAGCATGTGCTGAATTCTTG |
| Gli1 | Mouse | GGAAGTCCTATTCACGCCTTGA | CAACCTTCTTGCTCACACATGTAAG |
| GAPDH | Human | GTCAGTGGTGGACCTGACCT | TGCTGTAGCCAAATTCGTTG |
| GAPDH | Mouse | TCCCACTCTTCCACCTTCGATGC | GGGTCTGGGATGGAAATTGTGAGG |
| SHH siRNA_001 | CCAGAAACTCCGAGCGATT | ||
| SHH siRNA_002 | CTCCGAGCGATTTAAGGAA | ||
| SHH siRNA_003 | GAAACTCCGAGCGATTTAA |