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. 2022 Mar 14;13(1):569–577. doi: 10.1080/21505594.2022.2047506

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Flow cytometry gating strategy of follicular helper T cells (Tfh) and follicular regulatory T cells (Tfr) using FACSDiva software (Becton Dickinson Biosciences, USA).

(a) Lymphocytes were gated (R1) according to their forward and side scatter properties. (b, c) CD4+ T cells were then selected by drawing (R2), then (R3) was set to identify the CD4+ T cells expressing CXCR5 (Tfh). (d) A dot plot showing the activated Tfh cells (CD4+CXCR5+ICOS+Foxp3-), resting Tfh (CD4+CXCR5+ICOS-Foxp3-), and Tfr cells (CD4+CXCR5+ICOS+Foxp3+).