Figure 4.

NIH-CoVnb-112 inhibition of SARS-CoV-2 variant pseudovirus following nebulization. HEK293 cells overexpressing human ACE2 were cultured for 24 hours with pseudotyped SARS-CoV-2 spike variant virus, pretreated with NIH-CoVnb-112 at different concentrations. Following 48 hours incubation, HEK293-ACE2 cells were analyzed by flow cytometry to quantify the fluorescence level. NIH-CoVnb-112 potently inhibited viral transduction for all variant spike pseudoviruses with the following EC50 values: Prototype (14.2 nM), Alpha (9.4 nM), Beta (15.8 nM), Gamma (17.6 nM), and Delta (14.5 nM), respectively. Curve fit parameters are detailed in Supplementary Table 3. Error bars represent the standard deviation of the mean values at each data point.