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. 2022 Jan 16;26(1):10–18. doi: 10.1080/19768354.2021.2023037

Table 1.

List of oligonucleotide primers used for real-time PCR analysis.

Gene(GeneBank ID) Primer sequence (5′→3′) Tm (℃) Product size (bp)
Cx37 NM_021654 Antisense: GAAGAAGTGGTCGTAGCA, Sense: AGGAGTAGAAGGGAAAGC 53.5 314
Cx40 NM_019280 Antisense: CAATCTTCCCGTTCACCT-3′, Sense: TCTCCCACATTCGTTACTG 53.1 213
Cx43 NM_012567 Antisense: AGAGCACTGACAGCCACA, Sense: TCCAAGGAGTTCCACCAA 52.9 156
β-actin NM_031144 Antisense: TCCTTCTGACCCATACC, Sense: TTTGTGCCTTGATAGTTCG 55.6 260