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. 2021 Dec 12;28(4):566–579. doi: 10.1111/cns.13776

FIGURE 4.

FIGURE 4

SerpinA3N decreases apoptosis both in vitro and in vivo. Primary neuronal cultures were subjected to OGD/R, with or without serpinA3N treatment. Analyses were performed 4 h after OGD/R. (A) Cell viability evaluated by CCK‐8 kit. (B) Western blot analysis of nNOS and phosphorylated p38 levels (C–D) Western blot analysis of apoptosis‐related proteins Bcl‐2, Bax, Caspase‐3, and cleaved Caspase‐3. N = 6–7. *, ***, and **** p < 0.05, 0.001, and 0.0001. Mice were injected with AAV‐null‐ZsGreen or AAV‐serpinA3N‐ZsGreen, and brain tissue lysates were harvested 30 h after t‐MCAO. Western blot analysis of protein expressions of (E) phosphorylated p38, Caspase‐3, and cleaved Caspase‐3 (F) Bax and Bcl‐2. N = 4. ** and ***, p < 0.01 and 0.001