FIGURE 5.

Identification of clusterin as a SerpinA3N‐interacting protein. (A) Co‐immunoprecipitation of clusterin‐α using an anti‐serpinA3N antibody. (B) Co‐immunoprecipitation of serpinA3N using an anti‐clusterin‐α antibody. (C) Immunostaining of serpinA3N and clusterin‐α in mouse brain 24 h after MCAO. (D) Mice were injected with AAV‐null‐ZsGreen or AAV‐serpinA3N‐ZsGreen, and clusterin‐α was detected with Western blotting. N = 4. ****p < 0.0001. (E) Primary neuronal cultures were subjected to OGD/R with or without serpinA3N treatment, and clusterin‐α was detected with Western blotting. N = 4. ****p < 0.0001