TABLE 3.
Primary antibodies used in this study
| Primary antibodies | Host | Company | Dilution | ||
|---|---|---|---|---|---|
| IF | WB | Co‐IP | |||
| SerpinA3N | Goat | R&D | 1:300 | 1:5000 | 1:50 |
| NeuN | Mouse | Millipore | 1:100 | ||
| GFAP | Mouse | Boster | 1:100 | ||
| Iba1 | Rabbit | Abcam | 1:1000 | ||
| S100b | Mouse | Boster | 1:100 | ||
| Clusterin‐α | Mouse | Santa Cruz | 1:100 | 1:500 | 1:50 |
| GFP | Mouse | Santa Cruz | 1:200 | ||
| Bcl2 | Rabbit | Beyotime | 1:1000 | ||
| bax | Rabbit | Proteintech | 1:1000 | ||
| Caspase‐3 | Rabbit | CST | 1:2000 | ||
| Cleaved caspase‐3 | Rabbit | CST | 1:500 | ||
| iNOS | Rabbit | Boster | 1:1000 | ||
| TNFα | Mouse | Boster | 1:200 | ||
| p‐p38 | Rabbit | CST | 1:1000 | ||
| nNOS | Rabbit | Boster | 1:1000 | ||
| Akt | Rabbit | CST | 1:2000 | ||
| p‐Akt | Rabbit | CST | 1:2000 | ||
| mTOR | Rabbit | CST | 1:1000 | ||
| p‐mTOR | Rabbit | CST | 1:1000 | ||
| pCREB | Rabbit | Santa Cruz | 1:500 | ||
| β‐actin | Mouse | Beyotime | 1:10000 | ||
| β‐tubulin | Mouse | Beyotime | 1:5000 | ||