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. 2021 Dec 16;43(3):2238–2252. doi: 10.3390/cimb43030157

Figure 1.

Figure 1

Cytochrome c oxidase (CIV) activities of NALCL and ALCL. (a,b) are the protocols for measuring CIV activities in NALCL and ALCL, respectively. Titrations: Cell, dig (digitonin, cell permeabilization), Ama (antimycin A, CIII inhibition), CCCP (oxidative phosphorylation uncoupling), As (ascorbate, maintaining TMPD in a reduced state), TMPD (reducing cytochrome c), c (cytochrome c integrity of outer mt-membrane), and Azd (azide, CIV inhibition). Blue plots indicate O2 concentration and red plots are the O2 consumption expressed per cell. (c) The total O2 consumption rate was baseline-corrected for autoxidation after inhibition of CIV. Results are expressed as means ± S.D. * denotes p < 0.05 compared to NALCL as determined by t-test.