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. 2021 Dec 16;43(3):2238–2252. doi: 10.3390/cimb43030157

Figure 2.

Figure 2

Mitochondrial respiration of NALCL and ALCL. (a,b) are the protocols for measuring respiration in NALCL and ALCL, respectively. Titrations: Cell, dig (digitonin, cell permeabilization), P and M (pyruvate and malate, non-phosphorylating N-LEAK respiration, N(GM)L(n)), ADP (N-OXPHOS capacity, N(GM)P), c (cytochrome c integrity of outer mt-membrane), G (glutamate, N-OXPHOS capacity, N(PGM)P), S (succinate, NS-OXPHOS, NSP), CCCP (NS-ET capacity, NSE), Rot (rotenone, CI inhibition, S-ET capacity, SE), and Ama (antimycin A, CIII inhibition, Rox). Oxygen consumption was corrected for Rox. Blue plots indicate O2 concentration and red plots are the O2 consumption expressed per cell. (c) The Rox-corrected respiration: ROUTINE respiration, R, was measured in non-permeabilized cells in MiR05. After plasma membrane permeabilization, five respiratory states were sequentially established to measure NADH-linked LEAK respiration with pyruvate and malate, NL, OXPHOS capacity, NP, NS-pathway OXPHOS capacity, NSP, and NS- and S-pathway ET capacity, NSE and SE, where S indicates the succinate pathway. Results are expressed as means ± S.D. * denotes p < 0.05 while ** denotes p < 0.01 compared to NALCL as determined by t-test.