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. 2021 Dec 18;43(3):2289–2304. doi: 10.3390/cimb43030161

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Time-course profile of recombinant xylanase production in constructed P. pastoris strains during high-density fermentation in 3.6 L fermenter. (a) Enzymes activity of XynA; (b) protein concentration; (c) amount of biomass determined by OD₆₀₀DCW(g·L⁻¹); (d) yield per unit of biomass. The carbon-source-feed rate was 10%. The strains were respectively marked as (●) KM71/pGCW14-episomal, (▼) KM71/pGAP-integrated-pGCW14-episomal, (♦) KM71/pGCW14-integrated-pGCW14-episomal, and (▲) KM71/pGCW14-integrated-pGAP-episomal.