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. 2021 Oct 27;43(3):1794–1804. doi: 10.3390/cimb43030126

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Luminescent bioassay to measure cAMP following TSHR activation. Indirect assays to determine whether the TSHRv is functional were conducted using OF conditioned medium at various stages of differentiation (cells at D0 = Day 0 in complete medium, CM15 = complete medium for 15 days culture, DM15 = Differentiation medium for 15 days) in the presence of TSH/M22. Results are expressed as mean ± SEM; * p-value < 0.05, ** p-value < 0.01. Horizontal bars represent comparison between non-GO (grey) and GO (striped).