Figure 3.

Vismodegib inhibits the activation of the Hh pathway after PH. (A) QRT-PCR analysis of Hh signaling pathway intracellular signaling molecules GLI1 and GLI2 in liver tissue at different times after PH (0, 1, 2 and 3 days), n = 5. (B) Representative Western blot analysis of GLI1 and GLI2 in the liver tissue between the two groups at different times after PH (0, 1, 2 and 3 days). Western blot quantification analysis compared to GAPDH is shown under the bands. (C) Representative immunohistochemical pictures of GLI1 and GLI2 in the liver slices of mice at two days after PH between the two groups. (D) Results of GLI1 and GLI2 immunohistochemical quantitative analysis of the two groups at 2 days post-PH are shown in the histogram, analyzed with Image J, 5 pictures for each group, n = 3. All data represent means ± SEM. * p < 0.05, ** p < 0.01 vs. control group.