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. 2022 Jan 18;44(2):470–482. doi: 10.3390/cimb44020032

Figure 5.

Figure 5

Vismodegib increases the accumulation of polyploid cells through suppressing the degradation of Cyclin B. (A) QRT-PCR analysis of Cyclin B and Cdk1 in the liver tissue at different times after PH between the two groups (1, 2 and 3 days), n = 5. (B) Western blot analysis of Cyclin B, Cdc20 and Bub1b in liver tissue at different times after PH. Western blot quantification analysis compared to GAPDH is shown under the bands. (C) Quantification analysis of Cyclin B, CDC20 and BUB1B Western blot results is shown in the histogram. (D) Cell flow analysis of the proportion of polyploid primary hepatocytes by Hoechst 33342 in normal liver, and two groups of mice two days and three days after PH. The proportions of different polyploid cells are shown in the histogram. (E) H&E staining of liver slices at 3 days after PH showed different cell sizes between the two groups. A total of 100 cells were analyzed in each group. Cell sizes were analyzed by Image Pro Plus. Data represent means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group.