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. 2021 May 27;43(1):215–225. doi: 10.3390/cimb43010018

Figure 1.

Figure 1

Expression of inflammatory cytokines by astrocytes treated with ApoE isoforms. Human primary astrocytes were grown in vitro and treated for 24 h with 100 µM cholesterol (control) with or without recombinant ApoE2/3/4 (20 nM). Following treatment, cell lysate was collected and Western blot was used to assess the expression of inflammation-related proteins: (A) IL-1β, (B) TNFα, and (C) IL-6. N = 3, * p < 0.05 vs. Control, ** p < 0.01 vs. Control, *** p < 0.001 vs. Control, # p < 0.05 vs. ApoE3, ^ p < 0.05 vs. ApoE2, ^^ p < 0.01 vs. ApoE2.