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Expression of inflammatory cytokines by microglia treated with ApoE isoforms. Human immortalized microglia cells (HMC3) were grown in vitro and treated for 24 h with 100 µM cholesterol (control) with or without recombinant ApoE2/3/4 (20 nM). Following treatment, cell lysate was collected and Western blot was used to assess the expression of pro-inflammatory cytokines: (A) TNFα and (B) IL-6. N = 3, * p < 0.05 vs. Control.
