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. 2021 May 27;43(1):215–225. doi: 10.3390/cimb43010018

Figure 4.

Figure 4

Evaluation of neurotrophic factors following treatment with ApoE isoforms. Human primary astrocytes were grown in vitro and treated for 24 h with 100 µM cholesterol (control) with or without recombinant ApoE2/3/4 (20 nM). Following treatment, astrocyte supernatant was collected, concentrated 10-fold, and Western blot was used to assess the expression of secreted BDNF. Expression was evaluated for both the (A) proBDNF and (B) mature BDNF isoforms. N = 3, ** p < 0.01 vs. Control, *** p < 0.001 vs. Control, ### p < 0.001 vs. ApoE3, ^ p < 0.05 vs. ApoE2, ^^^ p < 0.001 vs. ApoE2.