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. 2022 Feb 11;44(2):889–927. doi: 10.3390/cimb44020060

Table 4.

Primers and PCR product sequence for the genus-specific detection of Curtobacterium.

Name Sequence Tm Product Size
Curto-F2 GAAATGGTGTTATGGCCGGAT 61.5 °C 275 bp
Curto-D-R ACGGGTTAACCTCGCCACA 61.5 °C
Product Sequence
GAAATGGTGTTATGGCCGGATGTGTATCCCAAGTAGCACGGGGCCCGAGAAATCCCGTGTGAATCTGTCAGGACCACCTGATAAGCCTAAATACTCCCAGATGACCGATAGCGGACAAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGGGAGGGGAGTGAAATAGTACCTGAAACCGTTTGCTTACAAACCGTCGGAGCCTCCTTGTAGGGGTGACGGCGTGCCTTTTGAAGAATGAGCCTGCGAGTTAGTGATATGTGGCGAGGTTAACCCGT