Figure 6.

Involvement of spinal noradrenergic system in the anti-allodynic effect of Tk. The effect of intrathecally injected prazosin (α₁-adrenergic-receptor antagonist) (A,B) and idazoxan (α₂-adrenergic-receptor antagonist) (C,D) on the effect of Tk. Changes in noradrenaline (NA) contents in the spinal cord following 1 h of Tk (500 mg/kg) oral administration (E). PBS was used as a control to prazosin, idazoxan and Tk (A–E). Control is mixture of 50% EtOH and 50% Cremophor EL solution (1:1) treated group as a control of Pacli + PBS (E). Pacli: paclitaxel, Tk: Trichosanthes kirilowii Maximowicz. Pacli + PBS + PBS: n = 6, Pacli + PBS + Tk: n = 6, Pacli + prazosin + Tk: n = 6, Pacli + PBS + PBS: n = 6, Pacli + PBS + Tk: n = 6, Pacli + Idazoxan + Tk: n = 6 (A–D). ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. baseline with two-way ANOVA followed by Sidak’s post-test for multiple comparisons. Control: n = 6, Pacli + PBS: n = 6, Pacli + Tk: n = 6 (E). ** p < 0.01 vs. Control and # p < 0.05 vs. Pacli + PBS with one-way ANOVA followed by Tukey’s post-test for multiple comparisons.