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Analysis of gene expression analysis for corneal epithelium markers (A,B) and limbal progenitor-related markers (C,D) in human iPS cells grown in a limbal cell culture system with varying durations of BMP4 administration. Human iPS cells were cultured in a limbal-specific medium (PI) with 10 ng/mL BMP4 during the first 1, 3, or 10 days of culture. Data represent 3 or 4 independent experiments and are presented as means ± standard deviation (* p < 0.05; ** p < 0.001; *** p < 0.001).
