Figure 1.

Schematic diagram showing the difference between northern blotting with the liquid hybridization methods. During northern blotting, extracted RNA is subjected to size fractionation using denaturing agarose or acrylamide gel. After fractionation, RNA is transferred to the positively charged or neutral membranes and crosslinked by UV or EDC (1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide), or baked, followed by hybridization with labeled probe(s) and detection. Contrary to this method, in the liquid hybridization technique, the RNA is initially hybridized with the labeled probe and then subjected to the non-denaturing agarose or acrylamide gel and subsequently transferred to the membrane, followed by its crosslinking/baking, and detection.