Figure 10.

Successful detection of pri- and pre-miRNAs using a probe in the pri-/pre-miRNA region of miR-17-92. (a) Schematic representation of miRNA structure highlighting its mature, pre-, and pri-miRNA regions and probe locations. Figure adapted from [47]. (b) Liquid hybridization of HC11 RNA (RNA1) with a probe outside the seed sequence (within the pri/pre-miRNA region) with (+) or without (−) Exo-I. The samples were run on a 6% non-denaturing acrylamide gel. The two bands at the bottom in the Exo-I (−) lanes are biotinylated primers that form primer/dimers in the absence of Exo-I and are degraded in its presence (Exo-I (+) lanes).