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. 2021 Oct 28;43(3):1828–1843. doi: 10.3390/cimb43030128

Figure 4.

Figure 4

Losartan suppressed inflammatory response and angiogenesis in EWAT. (A) Quantification of Il-1β, Il-6, Tgfβ, Tnfα, Ifnγ by qRT-PCR. qRT-PCR indicates quantification relative to Gapdh. (B) Quantification of NF-κB, pSTAT3, STAT3, and β-actin protein levels by Western blot of EWAT. Below graphs indicate quantification relative to Histone (for NF-κB) and STAT3 (for pSTAT3). (C) Representative TGFRβ2, VEGF, and MMP9 staining of EWAT. Red arrow highlights the positive staining. Scale bar: 100 μm. Tissue staining, as quantified by either stain intensity, is represented on the left-hand vertical axis in each graph. (D) Quantification of TGFβR2 and VEGF protein levels by Western blot of EWAT. Below graphs indicate quantification relative to β-actin (for TGFRβ2 and VEGF). For each animal group, n = 5. All values represent the mean ± SEM. Data were analyzed by Student’s t test. * p ≤ 0.05, normal vs. ob/ob; # p ≤ 0.05, ob/ob vs. ob/ob+ Losartan. EWAT: epididymal white adipose tissue; Il-6: interleukin-6; Tgfβ: transforming growth factor beta; Tnfα, tumor necrosis factor α; Ifnγ: interferon gamma; TGFβ: transforming growth factor beta; VEGF: vascular endothelial growth factor; MMP-9 matrix metallopeptidase 9.