Table 3.

Reaction setup for bacteria and viruses with final concentration.

Component	25 µL Reaction	Final Concentration
Luna Universal qPCR Master Mix	10 µL	1 X
Forward primer (10 µM)	1 µL	0.4 µM
Reverse primer (10 µM)	1 µL	0.4 µM
Template DNA	Variable	<100 ng
Nuclease-free Water	to 25 µL