Figure 1.

Study outline and sample workflow. (A) BC patients donated urine and blood samples prior to BC surgery and at an early and a late postsurgery check-up. Not all patients had a late postsurgery sample included. (B) Workflow from sample collection to EV quantification and small RNA sequencing. (C) The study consisted of two sequencing sets. Sequencing set 1 included urine EVs, serum EVs, and serum supernatant, while sequencing set 2 was limited to urine EVs and serum supernatant. Samples from ten T1 patients were run as replicas in sequencing set 2.