Figure 6.

(A) Photomicrographs representative of the pattern of collagen deposition in the experimental groups. On day 7, all groups showed deposition of thin and delicate fibrils of type III collagen in a predominantly reticular pattern. On day 14 CTR shows persistence of high content of type III collagen fibers (with green birefringence pattern) interspersed with some delicate type I collagen fibers (with yellow to red birefringence pattern), particularly in the bottom of the wounds. HEVL100 and 200 show predominance of reticular-arranged type I collagen fibers, whereas in HEVL 300 the fibers are thicker and assume a parallel arrangement (Sirius red/polarization, 100×). (B) Assessment of collagenization (percentage of collagen fibers content/0.025 mm²) within the healing areas in the experimental groups in day 7. Data are expressed as mean ± standard error of the mean. Significant differences in relation to the CTR (control) group are expressed as * p < 0.05 and ** p <0.01 (ANOVA and Tukey’s multiple comparison test).