Table 3.

Histological parameters assessed to calculate f wound healing state.

Histological Parameter	Scoring System	Microscopy (Histochemical Technique)
Amount of granulation tissue	profound-1, moderate-2, scanty-3, absent-4	Conventional light microscopy (HE stained)
Inflammatory infiltrate	plenty-1, moderate-2, a few-4	Conventional light microscopy (HE stained)
Collagen fiber orientation	vertical-1, mixed-2, horizontal-4	Polarized light microscopy (Sirius red stained)
Pattern of collagen	reticular-1, mixed-2, fascicle-4	Polarized light microscopy (Sirius red stained)
Amount of early collagen	profound-1, moderate-2, minimal-3, absent-4	Polarized light microscopy (Sirius red stained)
Amount of mature collagen	profound-1, moderate-2, minimal-4	Polarized light microscopy (Sirius red stained)