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(a) SDS-PAGE of the purified recombinant GST-peptide fusion product (2 µg). The band at about 29 kDa corresponds to the expected product while the band at about 25 kDa represents free GST. The amount of GST-peptide fusion product is equal to 3.2 mg per liter of culture. (b) SDS-PAGE (12.5%) of recombinant C-15867 after cleavage with thrombin (2 µg). The peptide yield is 1.8 mg per liter of culture.
