Figure 2.

Effect of PKC isozyme-selective inhibitors on histamine-induced upregulation of H1R gene expression in U-373 MG cells. Isozyme-selective PKC inhibitors were treated 1 h before stimulation with 100 μM histamine. After stimulation, total RNA was isolated, and the H1R mRNA level was determined by real-time RT-PCR. Inhibitors used are as follows: Ro-31-8220 (pan-PKC inhibitor), Go6976 (PKCα/β1 inhibitor), rottlerin (PKCδ inhibitor), and Ly333531 (PKCβ inhibitor). Data are represented as means ± SEM (n = 9). ** p < 0.01 vs. histamine. For statistical analysis, one-way ANOVA with Tukey–Kramer’s test was used.