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Time course of TDI-induced H1R mRNA upregulation in trigeminal ganglion (A) and nasal mucosa (B) of TDI-sensitized rats. At given time intervals after provocation with TDI, total RNA was isolated from trigeminal ganglion and nasal mucosa of TDI-sensitized and control rats. H1R mRNA was determined by real-time quantitative RT-PCR. Closed circles, TDI-sensitized rats; closed squares, control rats. Data are expressed as means ± SEM (n = 4).
