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Expression of H/I/K and B/K splice variants in U-373 MG cells and HeLa cells. After starvation in serum-free medium for 24 h, total RNA was isolated. Total RNA (2 μg) was reverse-transcribed to cDNA in the reaction mixture of 20 μL. Three μL of aliquot was used for the endpoint-PCR analysis by using splice variant-specific primers shown in Materials and Methods.
