Figure 7.

Time course of histamine-induced upregulation of H1R mRNA derived from B/K splice variant in U-373 MG cells (A) and HeLa cells (B). At given time intervals after stimulation with 100 μM (U-373 MG cells) or 10 μM (HeLa cells) histamine, total RNA was isolated, and B/K splice variant mRNA was determined by real-time quantitative RT-PCR using B/K specific primer and probe shown in Materials and Methods. Closed circles, B/K splice variant; closed squares, control. Data are expressed as means ± SEM (n = 6–9).