Table 1.
Oligonucleotide primers and probes used for real-time quantitative RT-PCR.
| Sequence |
|---|
| H1R mRNA Forward primer: 5’-CAGAGGATCAGATGTTAGGTGATAGC-3’ Reverse primer: 5’-AGCGGAGCCTCTTCCAAGTAA-3’ Probe: 5’-FAM-CTTCTCTCGAACGGACTCAGATACCACC-TAMRA-3’ |
| A/K variant mRNA Forward primer: 5’- AGCCCTGAGGTCTGGAGACAG -3’ Reverse primer: 5’- TTGCCCTCACAACATCTTGTCTTC -3’ Probe: 5’- FAM-TGAAACCAGCCAGGGAGTGAGCCATAC -TAMRA-3’ |
| B/K variant mRNA Forward primer: 5’-CAAACTTTCCCCGGAGCCG-3’ Reverse primer: 5’-TGTTGCCCTCACACATCTTTGTCTTC-3’ 1 Probe: 5’-FAM-CTCCTGCCT-3’ |
| F/K variant mRNA Forward primer: 5’-TTGCCAGGGTAAGAGGATGAG-3’ Reverse primer: 5’-AGGAATTGGGGAGGCTCATTG-3’ Probe: 5’-FAM-CAGGAGAGCAGCATTTGTAAAGGGAG-TAMRA-3’ |
|
Photinus pyralis luciferase (Pluc) mRNA Forward primer: 5’-TGAGTACTTCGAAATGTCCGTTC-3’’ Reverse primer: 5’-GTATTCAGCCCATATCGTTTCAT-3’ 2 Probe: 5’-FAM-GGCAGAAG-3’ |
|
Renilla reniformis luciferase (Rluc) mRNA Forward primer: 5’-GGAGAATAACTTCTTCGTGGAAAC-3’ Reverse primer: 5’-GCTGCAAATTCTTCTGGTTCTAA-3’ 3 Probe: 5’-FAM-TGTTGCCA-3’ |
1 Roche Universal Probelibrary probes #51. 2 Roche Universal Probelibrary probes #29. 3 Roche Universal Probelibrary probes #145.