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. 2021 Jul 28;163(4):753–764. doi: 10.1097/j.pain.0000000000002421

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Copyright © 2021 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain.

This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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Figure 5. — mPRMT7 promoted the hyperexcitability of mouse DRG neurons in a SCN11A-dependent manner. Current-clamp responses to 200-ms depolarizing current steps of 50, 100, 150, and 225 pA in representative Scn11a ^−/− mouse DRG neurons (A-B) and Scn11a ^+/+ mouse DRG neurons (C-D) expressing the empty vector pcDNA3.1/GFP (mock) and pcDNA3.1-PRMT7/GFP (PRMT7). (E) Rheobase, (F) resting membrane potential (RMP), and (G) V_threshold (the threshold at which AP takeoff occurs) were not significantly altered in mock-transfected DRG neurons. Data were statistically analysed by the unpaired Student t test; *P < 0.05 compared with the mock group. (H) Comparison of the average spike number of repetitive action potentials (APs) fired in response to the 200-ms current injection ranging from 0 to 225 pA in DRG neurons overexpressing the empty vector or PRMT7. (mNa_V1.9^+/+ + mock, n = 28; mNa_V1.9^+/+ + mPRMT7, n = 27; mNa_V1.9^−/− + mock, n = 41; mNa_V1.9^−/− + mPRMT7, n = 37). Significant differences were tested by two-way ANOVA, followed by a post hoc Bonferroni test; infection × current: F(27, 1027) = 1.815, P = 0.0069; infection: F(3, 1027) = 42.19, P < 0.0001; current: F(9, 1027) = 114.5, P < 0.0001; *P < 0.05 and **P < 0.01 compared with the mock group. ANOVA, analysis of variance; DRG, dorsal root ganglion; PRMT7, protein arginine methyltransferase 7.