FIG 3.

Time course of IT histone H4. Mice received either saline or 50 μg of histone H4 via IT instillation and sacrificed at 6, 12, or 24 h after IT. Mouse weight was recorded prior to IT instillation and at sacrifice, and the percent of original weight was calculated (A). BAL was collected and the cellular components analyzed for cell count and differential by cytospin to produce the fraction of PMNs (B) and the absolute number of PMNs (C) in the BAL. BAL fluid was then analyzed for total protein (D) and LDH (E). All individual data points are shown, with a line connecting the means. Black circles represent histone treatment, while gray circles represent saline. Albumin (F) was also measured from the 24-h BALs. For all graphs (except F), a two-way ANOVA was performed with the P value corresponding to the effect of histone treatment reported, and Bonferroni’s posttests were performed. In F, an unpaired t test was performed with P value reported. Data were from two independent experiments that totaled n = 6 mice per group. *, P < 0.05; **, P < 0.01; ***, P < 0.001.