FIG 6.

Effects of H4 combined with LPS at 12 h. Mice received either 50 μg of LPS or both 50 μg of H4 and 50 μg of LPS via IT instillation and sacrificed 12 h later. Mouse weight was recorded prior to IT instillation and at sacrifice, and the percent of original weight was calculated (A). BAL was collected and the cellular components analyzed for cell count and differential by cytospin to produce the fraction of PMNs (B) and the absolute number of PMNs (C) in the BAL. BAL fluid was then analyzed for total protein (D), LDH (E), citrullinated H3 as a marker of NETs (F), and albumin (G). Data shown as individual data points with the mean and standard error represented by solid lines. For all graphs, an unpaired t test was performed with P value reported. Data were from two independent experiments that totaled n = 5 mice per group.