FIG 7.

Effects of H4 blockade during pneumonia. Mice were infected with 1 to 2 million CFU of Klebsiella pneumoniae with either 50 μg of anti-histone H4 antibody or IgG1, κ isotype via IT instillation and sacrificed 24 h later. Mouse weight was recorded prior to IT instillation and at sacrifice, and the percent of original weight was calculated (A). BAL was collected and the cellular components analyzed for cell count and differential by cytospin to produce the fraction of PMNs (B) and the absolute number of PMNs (C) in the BAL. BAL fluid was then analyzed for total protein (D) and LDH (E). Data shown as individual data points with the mean and standard error represented by solid lines. Data were from two independent experiments that totaled n = 6 mice per group.