Figure 1.

Schematic overview of the experimental design used in the current study. Prx1 and Hic1 reporter mice were induced with 4 consecutive doses of tamoxifen to induce the expression of tdTomato. Mice were sacrificed at 1-, 2-, or 4-week post-induction, and the spines were processed with histological and immunohistochemical analysis (A). Prx1 and Hic1 mice carrying a DTA transgene were induced with 10 consecutive doses of tamoxifen to specifically ablate the Prx1- and Hic1-positive cell populations. Mice were sacrificed at 11 days after the first tamoxifen injection and the spines were processed with histological and immunohistochemical analysis (B). Prx1 and Hic1 reporter mice were induced with 4 consecutive doses of tamoxifen to induce the expression of tdTomato. One week after the last tamoxifen injection, the mice underwent a dura injury. Mice were sacrificed at 2-week post-injury and the spines were processed with histological and immunohistochemical analysis (C). Abbreviations: DTA, diphtheria toxin subunit A; Hic1, hypermethylated in cancer 1; Prx1, paired related homeobox-1.