Figure 4.

Hic1 protein expression in Prx1^{CreERT2−GFP+/+}R26R^tdTomato+/+ mice (A-C) and Prx1 protein expression in Hic1^CreERT2+/+R26R^tdTomato+/+ mice (D-F) 1-, 2-, and 4-week post-tamoxifen induction. The dura mater is outlined by the dashed line while the epidural fat is encircled by the solid line. Arrows indicate examples of colocalization between Prx1 and Hic1. Quantification of percentage of co-localized staining between Prx1⁺ with anti-Hic1 staining and Hic1⁺ with anti-Prx1 staining within the dura mater and epidural fat (G). An n = 3 mice were used per group per time point. ∗P < .05. Scale bars = 100 µm. Abbreviations: Hic1, hypermethylated in cancer 1; Prx1, paired related homeobox-1.