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. 2022 Feb 19;11(2):159–177. doi: 10.1093/stcltm/szab010

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© The Author(s) 2022. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Drug impacts on retinal ganglion cells of WT3-derived retinal organoids. (A-C): Expression of SNCG (A), HuC/D (B), and SMI-32 (C) identifying retinal ganglion cells. Photoreceptors are immunostained with recoverin (A-C). SNCG- (green; A) and HuC/D-positive cells (green, B) and SMI-32 immunofluorescence positive dendrites/axons were found in the center of retinal organoids while photoreceptors (Recov, red; A-C) formed a layer at the apical edge of retinal organoids at day 150 of differentiation. Nuclei were counterstained with Hoechst (blue). Scale bars, 20 μm. (D): Immunofluorescence quantification of SNCG and HuC/D for all conditions, showing a significant reduction of HuC/D-positive cells after digoxin and thioridazine exposure, while this effect for SNCG was only seen after digoxin treatment. Data are shown as mean ± SEM, N = 2 (independent experiments), and 5-20 images from different organoids were quantified per condition. Differences were considered statistically significant at ∗P < .05, ∗∗P < .01 and ∗∗∗P <.001. Hoe, Hoechst; Recov, recoverin.