FIG. 3.

Nucleotide and predicted amino acid sequences of orfX genes of NCTC8325 and orfX* genes of N315. The nucleotide sequence of the orfX gene of NCTC8325 located on the plasmid pLE1a was sequenced and compared to orfX* of N315. A possible integration site of mec DNA is indicated by a dotted arrow; the nucleotide position was ambiguous but within four nucleotides. Arrows indicate the locations and directions of primers used for the PCR assay. The accession number for the sequence of pLE1a is given in the Materials and Methods section.