Figure 1.

Overview of neoantigen prediction, prioritization, and validation. Neoantigen prediction relies on sample acquisition, high quality sequencing data, variant calling, and variant annotation. Neoantigen prioritization requires predicting some combination of the potential for the neoantigen to be expressed, processed, bound by MHC, and recognized by the T cell receptor (TCR). The development of neoantigen prioritization models relies on the availability of validated datasets of neoantigen immunogenicity. Figure created with BioRender.com.