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. 2022 Mar 17;17(3):e0265596. doi: 10.1371/journal.pone.0265596

Fig 1. Shows effect of hepcidin 1 on canine OC differentiation.

Fig 1

(A) The cells were treated with various concentrations of hepcidin followed by M-CSF (20 ng/mL) and RANKL (50 ng/mL) for 7 days. The cells were stained for TRAP stain and TRAP-positive cells (≥3 nuclei) were counted. Scale bar- 200 μm. (B) Bar graphs show the number of OC cells/well. Data are representative of three independent experiments and expressed as means ± SE. Means with *are significantly different from 0 μg/mL of hepcidin (*p < 0.05, **p < 0.01).