Skip to main content
. 2022 Mar 17;17(3):e0265486. doi: 10.1371/journal.pone.0265486

Fig 3. qPCR shows no difference in acute osteoclast-activity between Tcrd-H2BeGFP, Tcrd–/–and IL17af–/–mice, while RANKL/OPG ratio suggests an osteoclastogenic milieu in IL17af–/–mice.

Fig 3

The ligature-induced induction of IL-17 (A), IL-23 (B), GAS6 (C), TRAP (D), RANKL (E) and OPG (F) expression in Tcrd-H2BeGFP, Tcrd–/–and IL17af–/–mice measured by qPCR-analysis employing the ΔΔCt method and RANKL/OPG ratio (G) as a measure of osteoclastogenic milieu. In all diagrams each point represents a mouse. The data are normalized to HPRT-induction by ligature-induced periodontitis. Data were obtained from two independent experiments and statistically analyzed by Kruskal-Wallis test and Dunn´s multiple comparisons, two outliers were removed by Grubb´s test, mean and SD are shown. A p‐value below 0.05 was considered as significant.