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. 2022 Mar 17;17(3):e0264321. doi: 10.1371/journal.pone.0264321

Table 2. Mean daily protein and amino acid intakes by UPLC over 12 weeks in kittens supplemented with additional choline at 300 mg/kg BW0.75 (CHOLINE, n = 8) compared to a control group (CONTROL, n = 7) fed only the base diet.

CHOLINE (n = 8) CONTROL (n = 7) P Values
LSM SEM LSM SEM PGroup PTime PGroup*Time
Protein Intake (g/kg BW/d) 6.90 0.06 7.47 0.06 <0.0001 <0.0001 0.989
Alanine (g/kg BW/d) 0.45 0.01 0.48 0.01 <0.0001 <0.0001 0.996
Arginine (g/kg BW/d) 0.48 0.01 0.51 0.01 <0.0001 <0.0001 0.994
Aspartic Acid (g/kg BW/d) 0.61 0.02 0.66 0.02 <0.0001 <0.0001 0.996
Glutamine (g/kg BW/d) 0.96 0.03 1.04 0.03 <0.0001 <0.0001 0.996
Glycine (g/kg BW/d) 0.67 0.02 0.72 0.02 <0.0001 <0.0001 0.996
Histidine (g/kg BW/d) 0.16 0.005 0.17 0.005 <0.0001 <0.0001 0.996
Isoleucine (g/kg BW/d) 0.27 0.008 0.29 0.009 <0.0001 <0.0001 0.996
Leucine (g/kg BW/d) 0.50 0.02 0.54 0.02 <0.0001 <0.0001 0.996
Lysine (g/kg BW/d) 0.41 0.01 0.44 0.01 <0.0001 <0.0001 0.996
Phenylalanine (g/kg BW/d) 0.31 0.01 0.33 0.01 <0.0001 <0.0001 0.989
Proline (g/kg BW/d) 0.49 0.01 0.53 0.02 <0.0001 <0.0001 0.996
Serine (g/kg BW/d) 0.29 0.01 0.32 0.01 <0.0001 <0.0001 0.996
Threonine (g/kg BW/d) 0.27 0.01 0.29 0.01 <0.0001 <0.0001 0.996
Tyrosine (g/kg BW/d) 0.23 0.01 0.25 0.01 <0.0001 <0.0001 0.996
Valine (g/kg BW/d) 0.31 0.01 0.34 0.01 <0.0001 <0.0001 0.996
Methionine (g/kg BW/d) 0.15 0.004 0.16 0.005 <0.0001 <0.0001 0.996
Cysteine (g/kg BW/d) 0.25 0.008 0.28 0.008 <0.0001 <0.0001 0.996
Tryptophan (g/kg BW/d) 0.023 0.0001 0.025 0.0007 <0.0001 <0.0001 0.996
Taurine (g/kg BW/d) 0.046 0.001 0.049 0.001 <0.0001 <0.0001 0.996

LSM, least square means; SEM, standard error of the mean